Structure and properties of antimicrobial peptides produced by antagonist microorganisms isolated from Siberian natural objects

Introduction. Public healthcare urgently needs new pharmaceuticals – alternative to traditional antibiotics that pathogens develop no resistance to. Of special interest in this regard are antimicrobial, ribosomally synthesized bacterial peptides or bacteriocins. In work, we aimed study the structure and properties of antimicrobial produced by antagonist microorganisms isolated from natural objects Siberian region.
 Study methods. The were bacteria sources Kuzbass. After culturing bacteria, total protein was precipitated culture fluid separated into fractions gel permeation HPLC. Their amino acid sequences determined MALDI-TOF mass spectrometry. antibacterial (against Bacillus pumilus Escherichia coli) fungicidal Aspergillus flavus niger) studied disk diffusion method.
 Results discussion. Seven with different five which had analogues PepBank Uniprot data banks. peptide an sequence VMCLARKCSQGLIVKAPLM (2061.66 Da) homologous cysteine membrane Giardia lamblia P15, AVPSMKLCIQWSPVRASPCVMLGI (2587.21 showed a homology Planctomycetes bacterium I41 peptides. We found gram-positive gram-negative bacteria) Aspergillus) fractions.
 Conclusion. Antimicrobial region can be used create as treat infectious diseases.